DetaiBio possesses a mature protein expression system and advanced protein expression technology. Our fermentation services include normal fermentation, high-density fermentation, and process optimization. We have a variety of fermentation tanks, bioreactors and protein purification systems, which can provide recombinant proteins from milligram level to gram level. The expression system and fermentation protocol can be customized according to customers’ specific needs.
|Normal fermentation||5-50 mg/L||
|High-density fermentation||20-400 mg/L||3 weeks|
|Process optimization||100-1000 mg/L||
Gene sequence is a key factor in the success of protein expression. The MaxCodon™ bioinformatics software developed by DetaiBio can effectively optimize the genes (such as mRNA structure, rare codon, ribosomal binding site, etc.).
We optimize protein expression conditions, including temperature, inducer, OD and etc., leading higher cell culture density and protein expression in subsequent fermentation.
Depending on the different requirements of customers, DetaiBio provides normal fermentation and high-density fermentation. We optimize the fermentation process, including temperature, pH, dissolved oxygen, feeding mode and so on. In addition, fed-batch strategy is the most effective method to achieve a high yield for high-density fermentation.
After obtaining a large amount of protein-expressing bacterial sludge, we can provide the corresponding protein purification service, among which a variety of protein purification methods are included to provide customers with the desired purity of the protein.
The fed-batch strategy is an effective method to achieve high yield recombinant protein in high-density fermentation. The quality and productivity of cells can be maximized by adjusting the culture conditions, including temperature, PH value and the feed rate of culture and substrate.
For quote, please fill in the online form and email to Sales@Detaibio.com.
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