DetaiBio provides E.coli guaranteed protein expression service. You only needs to provide the gene or protein sequence of target protein and DetaiBio will deliver the agreed quantity and purity of target protein according to contract. If unsuccessful, we will not charge any fees (including gene synthesis).
|Gene Synthesis and Subcloning||Codon optimization and gene synthesis||
|Transformation and Expression Optimization||The constructed expression plasmid was transformed into E. coli|
|Test and optimize the expression conditions|
|Pilot-Scale Expression and Purification||According to the optimization conditions, amplification and multi-step purification are performed to obtain the target protein purification.|
|Protein Refolding||If the expressed protein is inclusion body, then a number of refolding methods are performed to obtain the soluble target protein|
Additional Services: We also provide tag-free protein expression and purification, (Tag-Free), Large-scale production, and endotoxin control and removal service.