We are proud of our ability to offer you recombinant protein production service. We have rich experience in the expression of recombinant proteins (antibodies, enzymes, membrane proteins, cytokines, etc.) in any species. DetaiBio provides protein expression and purification services from small-scale to large-scale. If you can’t find your protein of interest on the market, please contact us to discuss your project and create a custom quote.?
Currently, there are four widely used cell based expression systems: mammalian, bacterial (E.coli), yeast and insect cells. We offer four systems for protein expression, click on the system you are interested in to see a breakdown of the steps involved with approximate pricing.
Bacteria have no nucleus, endoplasmic reticulum, or golgi apparatus, which are all key elements in cellular transport and post translational modification. In contrast, eukaryotes like mammalian cells possess these organelles and the molecular machinery that come with them.
There are two ways for protein expression of mammalian cells: transient transfection and stable transfection. HEK293 or CHO cells are often used in transient transfection. A small amount of protein can be quickly obtained within one week after the transient transfection. Meanwhile, there are dozens of cells that can be stably transfected, which can be used to produce cell lines after stable transfection and screening. Stable cell lines can grow continuously over a long period of time without significant change in the expression level of the target gene.
E.coli is the most commonly used protein expression system, for its advantages of fast growing, high protein expression level, and low cost (compared to mammalian expression system). We guarantee that if the E.coli protein expression fails, there will be no charge for each step from gene synthesis to protein purification. However, the cell wall of?E.coli?contains toxic pyrogens and the expressed proteins may need to be extensively tested before use.
Purification of the target protein from all impurities is a challenging step. We are equipped with advanced protein purification systems and a thoughtful team. We have mastered the principles and processes of the four major protein purification methods, including affinity chromatography, ion-exchange, size-exclusion and hydrophobic interaction. At present, we have successfully purified a variety of proteins, such as polyproteins, toxic proteins, and easily degradable proteins.
We can provide specific tags during vector construction, which is helpful for purification. Commonly available tags are His, GST, SUMO, MBP, Fc, etc. During purification, we will also control the endotoxin content to a concentration of 0.1-1.0 EU/ug. The concentration of finally delivered protein is also up to you.
For the selection of protein expression systems, different expression systems vary in terms of their advantages, disadvantages, and applicability. The properties and application of the protein should be considered when choosing the optimal protein expression system. A simple comparison of the four expression systems is as follows:
|Turnaround||6-9 weeks||4-6 weeks||6-9 weeks||6-9 weeks|
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For quote, please fill in the online form and email to Sales@Detaibio.com.
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