DetaiBio provides one-stop recombinant antibody expression services with high high quality and efficiency. Using our self developed high expression vectors and improved expression systems, we can produce full-length antibodies or various types of antibody fragment from milligram level to gram level. Please contact us for quotations and detailed information.
Customers can provide any of the following three options:
||Sequencing report (if sequencing)||
|Subcloning and transfection||Antibody genes are constructed into mammalian expression vector, and then transfected into CHO / HEK293 cells.||1 μg constructed vectors with antibody gene||1 week|
|Transient expression & Purification||After transient expression, we can purify 1-3 mg recombinant antibody with purity more than 90% using Protein A/G column.||
|QC||ELISA and western blot is performed for detection.||Western blot and ELISA report||1 week|
At DetaiBio, we can sequence full-length antibody or variable region services. Using the 5’ RACE method, the complete sequences of an antibody or the variable region of an antibody are amplified, cloned and sequenced, which are subsequently cloned into two cloning vectors for expression vector constructions.
Transient expression can be used to express the antibodies in a few days, while stable cell line can produce large amounts of antibodies in the long-term. Both the two expression methods are able to obtain fully post-translational modified antibodies.
For quote, please fill in the online form and email to Sales@Detaibio.com.
Detai Bio-Tech Co.,Ltd ©2018 All Rights Reserved
No.10,XingHuo Road,High-tech Development District,Nanjing City,JiangSu Province,China Sales@DetaiBio.com