Recombinant Antibody Expression

DetaiBio provides one-stop recombinant antibody expression services with high high quality and efficiency. Using our self developed high expression vectors and improved expression systems, we can produce full-length antibodies or various types of antibody fragment from milligram level to gram level. Please contact us for quotations and detailed information.

Service Highlights

  • Guaranteed package: we offer guaranteed recombinant antibody expression service. No recombinant antibody delivered, no billing!
  • Comprehensive expression services: we have the ability to produce a wide range of antibody formats, such as full-length IgG, IgD, IgE, scFv/Fab, VHH, sdAb, chimeric antibody, etc.
  • One-stop service: our one-stop services include antibody sequencing, antibody expression (full-length or fragments), chimeric antibody expression, and antibody labeling. And all the steps can be customized according to customers’ requirements.
  • High success rate: DetaiBio has self built cGMP standard laboratory, self developed high expression vector proEM, and improved CHO / HEK293 expression systems, leading the high success rate.

Service Content

Procedure Description Deliverables Timeline
Customers provide Customers can provide any of the following three options:

  • hybridoma cells (sequenced by DetaiBio)
  • antibody sequences (synthesized by DetaiBio)
  • constructed vectors with antibody genes
Sequencing report (if sequencing)
  • 2-3 weeks for sequencing
  • 2 weeks for gene synthesis
Subcloning and transfection Antibody genes are constructed into mammalian expression vector, and then transfected into CHO / HEK293 cells. 1 μg constructed vectors with antibody gene 1 week
Transient expression & Purification After transient expression, we can purify 1-3 mg recombinant antibody with purity more than 90% using Protein A/G column.
  • 1-3 mg antibody with purity ≥ 90%
  • Experimental report
3 weeks
QC ELISA and western blot is performed for detection. Western blot and ELISA report 1 week

Notes:

  1. Quantity of recombinant antibodies can be customized (0.01-1 g). DetaiBio can also offer stable cell line for producing recombinant antibodies in long term.
  2. Recombinant antibodies can be labeled by biotin, isotope or fluorescein if required.
  3. DetaiBio has ability to generate a wide range of antibody formats, including full-length IgG, IgD, IgE, scFv, Fab, Fab’, VHH, sdAb, chimeric antibody, etc.
  4. We can also provide scFv/Fab construction services using phage display technology.
  5. During the entire experimental procedure, endotoxin is controlled to make sure the final level less than 1 EU/μg.
  6. If you have any questions, please contact us.

Supplementary

Hybridoma Sequencing

At DetaiBio, we can sequence full-length antibody or variable region services. Using the 5’ RACE method, the complete sequences of an antibody or the variable region of an antibody are amplified, cloned and sequenced, which are subsequently cloned into two cloning vectors for expression vector constructions.

Transient Expression & Stable Cell Line

Transient expression can be used to express the antibodies in a few days, while stable cell line can produce large amounts of antibodies in the long-term. Both the two expression methods are able to obtain fully post-translational modified antibodies.

Contact Us

For quote, please fill in the online form and email to Sales@Detaibio.com.

Name * :
Email * :
Message * :
Captcha * :
captcha

For Research Use Only
Top 野草社区在线高清视频_揉她的大白胸把她摸湿_国内精品久久久久影院